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rnaiso plus reagent  (TaKaRa)


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    Structured Review

    TaKaRa rnaiso plus reagent
    Rnaiso Plus Reagent, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 30964 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rnaiso+plus+reagent/RNAiso+Plus/pmc13011246-56-6-9
    Average 99 stars, based on 30964 article reviews
    rnaiso plus reagent - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Cell Culture:

    Article Title: In silico and in vitro evaluation of risedronate as a DKK1 antagonist: Implications for neuroprotection through DKK1-LRP6 modulation.
    Article Snippet: .. Afterwards, the RNAiso plus reagent (Cat. #9108) was used to isolate total RNA from the cell culture, following the instructions provided by the manufacturer (Zhu et al., 2018) (Takara Bio Inc). ..

    Article Title: IL-6 exacerbates calcium oxalate kidney stones by promoting inflammatory responses and crystal adhesion via p38 MAPK signaling activation
    Article Snippet: .. Total RNA was isolated from both cultured HK-2 cells and rat kidney outer medulla tissues using RNAiso Plus reagent (TAKARA, Cat# 9108). .. Complementary DNA (cDNA) was synthesized employing the PrimeScriptTM FAST RT reagent Kit with gDNA Eraser (TAKARA, Cat# RR092A) to remove potential genomic DNA contamination.

    Article Title: Melatonin Counteracts Mechanical Unloading‐Induced Bone Loss Through YTHDF3‐Mediated m 6 A Modification of Dapk2 mRNA
    Article Snippet: .. Total RNA was purified from tissue samples and cultured cells using RNAiso Plus reagent (TaKaRa, Japan) following the manufacturer's specifications. .. Reverse transcription was performed with PrimeScript RT Master Mix (TaKaRa, Japan) to generate complementary DNA templates. qRT–PCR amplification was conducted using SYBR Premix Ex Taq II on a CFX96 Real‐Time PCR Detection System (Bio‐Rad, USA).

    Reverse Transcription:

    Article Title: Mpox virus D8L protein binds to STAT1 and inhibits its phosphorylation to antagonize IFN-induced signaling.
    Article Snippet: .. Total RNA was extracted using RNAiso Plus reagent ( Cat# 9109; Takara) after 24 h, and RNA was reverse transcribed into cDNA using PrimeScript FAST RT reagent (Cat# RR092A; Takara), and real-time PCR was performed using TB Green Premix Ex Taq II FAST qPCR reagent (Cat# CN830A; Takara). ..

    Article Title: HCMV-pUS2 Disrupts cGAS-STING Signaling through LMAN2L Degradation
    Article Snippet: .. Total RNA was extracted from 5 × 105 cells using RNAiso Plus reagent (cat. no. 9109; TaKaRa) and reverse-transcribed into cDNA. ..

    Real-time Polymerase Chain Reaction:

    Article Title: Mpox virus D8L protein binds to STAT1 and inhibits its phosphorylation to antagonize IFN-induced signaling.
    Article Snippet: .. Total RNA was extracted using RNAiso Plus reagent ( Cat# 9109; Takara) after 24 h, and RNA was reverse transcribed into cDNA using PrimeScript FAST RT reagent (Cat# RR092A; Takara), and real-time PCR was performed using TB Green Premix Ex Taq II FAST qPCR reagent (Cat# CN830A; Takara). ..

    Isolation:

    Article Title: IL-6 exacerbates calcium oxalate kidney stones by promoting inflammatory responses and crystal adhesion via p38 MAPK signaling activation
    Article Snippet: .. Total RNA was isolated from both cultured HK-2 cells and rat kidney outer medulla tissues using RNAiso Plus reagent (TAKARA, Cat# 9108). .. Complementary DNA (cDNA) was synthesized employing the PrimeScriptTM FAST RT reagent Kit with gDNA Eraser (TAKARA, Cat# RR092A) to remove potential genomic DNA contamination.

    Agarose Gel Electrophoresis:

    Article Title: Knockout of bsal/cel.2 results in growth retardation, reduced lipid digestion and altered energy metabolism in medaka larvae (oryzias latipes).
    Article Snippet: 1 College of Fisheries, Chinese Perch Research Center, Huazhong Agricultural University, Wuhan 430070, China 2 Engineering Research Center of Green development for Conventional Aquatic Biological Industry in the Yangtze River Economic Belt, Ministry of Education, Wuhan 430070, China 3 College of Fisheries, Huazhong Agricultural University, No.1, Shizishan Street, Hongshan District, Wuhan 430070, Hubei Province, China Abstract To evaluate the significance of bile salt-activated lipase (BSAL) in lipid digestion and metabolism in fish, this study used CRISPR/Cas9 gene editing to generate medaka (Oryzias latipes) bsal mutant lines.. Given that the bsal gene comprises three copies (bsal, cel.2, and bsal-like) in the medaka genome, bsal-like variants may lead to functional loss in lipid hydrolysis owing to amino acid residue alterations in the bile salt binding site.. Consequently, two types of medaka mutants, bsal−/− and bsal−/−/cel.2−/−, were generated for experimental exploration in this study.

    Spectrophotometry:

    Article Title: Knockout of bsal/cel.2 results in growth retardation, reduced lipid digestion and altered energy metabolism in medaka larvae (oryzias latipes).
    Article Snippet: 1 College of Fisheries, Chinese Perch Research Center, Huazhong Agricultural University, Wuhan 430070, China 2 Engineering Research Center of Green development for Conventional Aquatic Biological Industry in the Yangtze River Economic Belt, Ministry of Education, Wuhan 430070, China 3 College of Fisheries, Huazhong Agricultural University, No.1, Shizishan Street, Hongshan District, Wuhan 430070, Hubei Province, China Abstract To evaluate the significance of bile salt-activated lipase (BSAL) in lipid digestion and metabolism in fish, this study used CRISPR/Cas9 gene editing to generate medaka (Oryzias latipes) bsal mutant lines.. Given that the bsal gene comprises three copies (bsal, cel.2, and bsal-like) in the medaka genome, bsal-like variants may lead to functional loss in lipid hydrolysis owing to amino acid residue alterations in the bile salt binding site.. Consequently, two types of medaka mutants, bsal−/− and bsal−/−/cel.2−/−, were generated for experimental exploration in this study.

    Purification:

    Article Title: Melatonin Counteracts Mechanical Unloading‐Induced Bone Loss Through YTHDF3‐Mediated m 6 A Modification of Dapk2 mRNA
    Article Snippet: .. Total RNA was purified from tissue samples and cultured cells using RNAiso Plus reagent (TaKaRa, Japan) following the manufacturer's specifications. .. Reverse transcription was performed with PrimeScript RT Master Mix (TaKaRa, Japan) to generate complementary DNA templates. qRT–PCR amplification was conducted using SYBR Premix Ex Taq II on a CFX96 Real‐Time PCR Detection System (Bio‐Rad, USA).

    RNA Extraction:

    Article Title: UBE2C promotes pancreatic cancer progression through PI3K/Akt/mTOR signaling pathway
    Article Snippet: .. RNA extraction was performed by adding 1 mL of RNAiso Plus reagent (Takara, 9109, China) to the cells, followed by thorough mixing. ..



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